This article focuses on reagents with purity 99 and labeled "for scientific research only", focusing on "scientific use in the whole process", and analyzes the key points of safe use from three core dimensions: first, standardize the operating process, covering laboratory environmental requirements, wearing protective equipment, accurate weighing and storage details of reagents of different specifications (10mg/20mg/30mg) to ensure reagent purity and experimental data accuracy; the second is to clarify the use boundaries, emphasize strict limitation of scientific research scenarios, eliminate misuse in non-scientific research fields, and take into account compliance and personnel safety; the third is to standardize waste treatment, classify and dispose of residual reagents according to standards, and avoid environmental and secondary safety risks. the article finally points out that safe use is a system behavior that runs through "before operation-during operation-after operation" and can realize multiple scientific research values of "data accuracy, personnel safety, and process compliance".